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human il33 (R&D Systems)

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R&D Systems human il33
Human Il33, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+il33/Human+IL-33+Antibody/us12594324-380-11-14
Average 93 stars, based on 88 article reviews

human il33 - by Bioz Stars, 2026-09

93/100 stars

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other:

Article Title: ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity.
Article Snippet: Detection was performed with a DAB detection kit (Ventana Medical Systems) according to the manufacturer’s instructions.

Enzyme-linked Immunosorbent Assay:

Article Title: WFDC2 Promotes Spasmolytic Polypeptide-Expressing Metaplasia Through the Up-Regulation of IL33 in Response to Injury.
Article Snippet: .. ELISA was performed for the respective samples with the use of human WFDC2 (no. LS-F39786; LSBio), mouse IL33 (no. M3300; R&D Systems), and human IL33 (no. D3300B; R&D Systems) kits in accordance with the manufacturers’ instructions. ..

Incubation:

Article Title: ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity.
Article Snippet: Sections were blocked for 30 min with Background Buster solution (Innovex), followed by avidin-biotin blocking for 8 min (Ventana Medical Systems). .. Mouse IL33 (AF3626, R&D Systems), mouse smooth muscle actin (Abcam), and human IL33 (AF3625, R&D Systems) antibodies were applied, and sections were incubated for 4 h, followed by a 60-min incubation with biotinylated rabbit anti-goat IgG (Vector labs), or biotinylated goat anti-rabbit IgG (Vector labs) at 1:200 dilution. .. Detection was performed with a DAB detection kit (Ventana Medical Systems) according to the manufacturer’s instructions.

Article Title: Methods and compositions for treatment of pancreatic cancer
Article Snippet: Sections were blocked for 30 minutes with Background Buster solution (Innovex), followed by avidin-biotin blocking for 8 minutes (Ventana Medical Systems). .. Mouse IL33 (AF3626, R&D Systems), mouse smooth muscle actin (Abcam), and human IL33 (AF3625, R&D Systems) antibodies were applied, and sections were incubated for 4 hours, followed by a 60-minute incubation with biotinylated rabbit anti-goat IgG (Vector labs), or biotinylated goat anti-rabbit IgG (Vector labs) at 1:200 dilution. .. Detection was performed with DAB detection kit (Ventana Medical Systems) according to the manufacturer's instructions.

Purification:

Article Title: Long-acting muscarinic antagonist regulates group 2 innate lymphoid cell-dependent airway eosinophilic inflammation.
Article Snippet: | 3 MATSUYAMA eT Al. 2.2 | Papain- induced airway inflammation Mice were anesthetized with isoflurane, treated with 25 μg papain (Calbiochem, San Diego, CA, USA) in 40 μl saline intranasally for 3 consecutive days, and sacrificed 24 h after the last papain administration.. Bronchoalveolar lavage fluids (BALF) and lung tissues were collected.. Total cell counts were determined by light microscopy, using a standard hemocytometer.

Co-Culture Assay:

Immunohistochemistry:

Article Title: WFDC2 Promotes Spasmolytic Polypeptide-Expressing Metaplasia Through the Up-Regulation of IL33 in Response to Injury.
Article Snippet: All of the sections were examined under a transmission electron microscope (Jeol) at 80 kV. .. The following primary antibodies were commercially purchased or were kindly provided by colleagues and used for immunohistochemistry, Western blot, and immunofluorescence analyses: human WFDC2 (no. orb101171; Biorbyt), MIST1 (no. 1489six, CST), MUC5AC (no. MA5-12178; Invitrogen), MKI67 (no. ab16667; Abcam), ATP4A (no. DO31-3; MBL), TFF2 (no. 13681-1-AP; Proteintech), GSII (no. L21415; Thermo), GIF (from Dr. David Alpers, Washington University), CD44v9 (no. LKG-M002; Cosmo Bio), F4/80 (no. ab6640; Abcam), CD163 (no. sc-58965; Santacruz), human IL33 (no. AF3625-SP; R&D Systems), mouse IL33 (no. AF3626; R&D systems), and E-cadherin (no. sc-59778; Santa Cruz Biotechnology). ..

Western Blot:

Immunofluorescence:

Image Search Results

(A) Crystal structure of IL1RAP (PDB: 4DEP), with IL1 (olive), IL-1RI (grey) and c2d2 loop (blue) interfaces colored. (B) Crystal structure of IL1RAP (PDB: 5VI4), with IL33 (cyan), IL-33R (pink) and c2d2 loop (green) interfaces colored. (C) Crystal structure of IL1RAP (PDB: 3O4O), with IL1 (dark goldenrod), IL-1R2 (deep pink) and c2d2 loop (red) interfaces colored. (D) Schematic diagram of antibody discovery and screening workflow. (E) ELISA binding assessment of the parental antibody to full-length IL1RAP and its individual domains.

Journal: bioRxiv

Article Title: Anti-IL1RAP Antibodies for Pan-Inhibition of IL-1 Family Cytokine Signaling in Inflammatory Diseases and Oncology

doi: 10.64898/2026.02.13.705739

Figure Lengend Snippet: (A) Crystal structure of IL1RAP (PDB: 4DEP), with IL1 (olive), IL-1RI (grey) and c2d2 loop (blue) interfaces colored. (B) Crystal structure of IL1RAP (PDB: 5VI4), with IL33 (cyan), IL-33R (pink) and c2d2 loop (green) interfaces colored. (C) Crystal structure of IL1RAP (PDB: 3O4O), with IL1 (dark goldenrod), IL-1R2 (deep pink) and c2d2 loop (red) interfaces colored. (D) Schematic diagram of antibody discovery and screening workflow. (E) ELISA binding assessment of the parental antibody to full-length IL1RAP and its individual domains.

Article Snippet: Cells were treated with escalating concentrations of DXP-006, DXP-106, or antagonists as indicated, followed by stimulation with IL33 (Sinobiological, 10368-HNAE) or IL1α+IL1β+IL33 at their respective pre-determined EC 80 .

Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

(A-B) Inhibition of IL1α (A) and IL1β (B) induced signaling by indicated antibodies in HEK-Blue IL-1 reporter cells. (C) Inhibition of IL33 induced signaling by indicated antibodies in HUVEC cells. (D) Inhibition of IL1α, IL1β, and IL33 induced signaling by indicated antibodies in HUVEC cells. (E-G) Inhibition of IL36α (E), IL36β (F) and IL36γ (G) induced signaling by indicated antibodies in A431 cells. (H) Inhibition of IL1α, IL1β, IL36α, IL36β, and IL36γ induced signaling by indicated antibodies in A431 cells.

Journal: bioRxiv

Article Title: Anti-IL1RAP Antibodies for Pan-Inhibition of IL-1 Family Cytokine Signaling in Inflammatory Diseases and Oncology

doi: 10.64898/2026.02.13.705739

Figure Lengend Snippet: (A-B) Inhibition of IL1α (A) and IL1β (B) induced signaling by indicated antibodies in HEK-Blue IL-1 reporter cells. (C) Inhibition of IL33 induced signaling by indicated antibodies in HUVEC cells. (D) Inhibition of IL1α, IL1β, and IL33 induced signaling by indicated antibodies in HUVEC cells. (E-G) Inhibition of IL36α (E), IL36β (F) and IL36γ (G) induced signaling by indicated antibodies in A431 cells. (H) Inhibition of IL1α, IL1β, IL36α, IL36β, and IL36γ induced signaling by indicated antibodies in A431 cells.

Techniques: Inhibition

(A) Cryo-EM map of the complex that contains DXP-006 (purple and pink), IL1RAP (cyan; domain 2 and domain 3) and density map (grey), c2d2 loop (blue density map). (B) Interfaces between DXP-006 and IL1RAP. (C) Crystal structure of IL1RAP with the DXP-006 Interface (cyan) and CAN10 interface (blue). (D) Crystal structure of IL1RAP domain 2 and domain 3 (PDB:4DEP), colored with IL-1 (yellow), IL33 (orange), IL-36 (green) and DXP-006 (cyan) interface.

Journal: bioRxiv

Article Title: Anti-IL1RAP Antibodies for Pan-Inhibition of IL-1 Family Cytokine Signaling in Inflammatory Diseases and Oncology

doi: 10.64898/2026.02.13.705739

Figure Lengend Snippet: (A) Cryo-EM map of the complex that contains DXP-006 (purple and pink), IL1RAP (cyan; domain 2 and domain 3) and density map (grey), c2d2 loop (blue density map). (B) Interfaces between DXP-006 and IL1RAP. (C) Crystal structure of IL1RAP with the DXP-006 Interface (cyan) and CAN10 interface (blue). (D) Crystal structure of IL1RAP domain 2 and domain 3 (PDB:4DEP), colored with IL-1 (yellow), IL33 (orange), IL-36 (green) and DXP-006 (cyan) interface.

Techniques: Cryo-EM Sample Prep

Journal: bioRxiv

Article Title: Anti-IL1RAP Antibodies for Pan-Inhibition of IL-1 Family Cytokine Signaling in Inflammatory Diseases and Oncology

doi: 10.64898/2026.02.13.705739

Techniques: Inhibition

Diabetic liver fibrosis is alleviated in interleukin 33 (IL33)-deficient mice. (A) Schematic diagram of the experimental procedure, mice were fed with normal diet (ND) or high-fat diet (HFD) for a total of 26 weeks. After 12 weeks, HFD-fed mice were consecutively injected with streptozotocin (STZ, 50 mg/kg) for 5 days. Diabetic phenotype was validated by fasting blood glucose ≥11.1 mmol/L at 7 days later (14 weeks). HFD-fed mice were consecutively injected with αIL33, immunoglobulin G (IgG), recombinant IL33 (rIL33), or vehicle for 12 weeks. (B) Serum IL33 level in control (Ctrl) mice and diabetic (DM) mice assessed by enzyme-linked immunosorbent assay (ELISA) ( n =12). (C) Western blot and measurement for IL33 expression of whole liver ( n =6). (D) Representative images of H&E and Sirius Red staining of liver sections from DM mice and IL33 knockout (KO) diabetic mice. Scale bar, 100 μm. (E) A positive area of Sirius Red staining is used to quantify liver fibrosis (right) ( n =4). (F) Hepatic hydroxyproline levels in mice ( n =4). (G, H) Representative images and quantify of immunohistochemical (IHC) staining of alpha smooth muscle actin (αSMA) in liver sections ( n =4). Scale bar, 100 μm. Data was shown as mean±standard error of the mean. ST2, suppression of tumorigenicity 2; i.p., intraperitoneal; PBS, phosphate buffered saline; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. a P <0.01.

Journal: Diabetes & Metabolism Journal

Article Title: Interleukin 33 Promotes Liver Sinusoidal Endothelial Cell Dysfunction and Hepatic Fibrosis in Diabetic Mice

doi: 10.4093/dmj.2024.0532

Figure Lengend Snippet: Diabetic liver fibrosis is alleviated in interleukin 33 (IL33)-deficient mice. (A) Schematic diagram of the experimental procedure, mice were fed with normal diet (ND) or high-fat diet (HFD) for a total of 26 weeks. After 12 weeks, HFD-fed mice were consecutively injected with streptozotocin (STZ, 50 mg/kg) for 5 days. Diabetic phenotype was validated by fasting blood glucose ≥11.1 mmol/L at 7 days later (14 weeks). HFD-fed mice were consecutively injected with αIL33, immunoglobulin G (IgG), recombinant IL33 (rIL33), or vehicle for 12 weeks. (B) Serum IL33 level in control (Ctrl) mice and diabetic (DM) mice assessed by enzyme-linked immunosorbent assay (ELISA) ( n =12). (C) Western blot and measurement for IL33 expression of whole liver ( n =6). (D) Representative images of H&E and Sirius Red staining of liver sections from DM mice and IL33 knockout (KO) diabetic mice. Scale bar, 100 μm. (E) A positive area of Sirius Red staining is used to quantify liver fibrosis (right) ( n =4). (F) Hepatic hydroxyproline levels in mice ( n =4). (G, H) Representative images and quantify of immunohistochemical (IHC) staining of alpha smooth muscle actin (αSMA) in liver sections ( n =4). Scale bar, 100 μm. Data was shown as mean±standard error of the mean. ST2, suppression of tumorigenicity 2; i.p., intraperitoneal; PBS, phosphate buffered saline; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. a P <0.01.

Article Snippet: Wild-type diabetic mice were given an intraperitoneal injection of anti-IL33 neutralizing antibody (αIL33, 37 μg/kg body weight, R&D Systems, Minneapolis, MN, USA) or recombinant IL33 (rIL33, 12.5 μg/kg body weight, R&D Systems) twice a week for last 12 weeks.

Techniques: Injection, Recombinant, Control, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing, Staining, Knock-Out, Immunohistochemical staining, Immunohistochemistry, Saline

Interleukin 33 (IL33) promotes liver sinusoidal endothelial cell (LSEC) dysfunction in diabetic mice. (A) Representative scanning electron micrographs of livers of mice. Scale bar, 5 μm. (B) Hepatic nitric oxide (NO) levels ( n =8). (C, D) Representative immunofluorescent images and analysis of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and CD31 of liver sections ( n =4) (scale bar, 100 μm). (E) Relative mRNA abundance of vascular cell adhesion molecule 1 (Vcam1), intercellular adhesion molecule 1 (Icam1), nitric oxide synthase 2 (Nos2), endothelin 1 (Edn-1), nitric oxide synthase 3 (Nos3), and KLF transcription factor 2 (Klf2) of liver tissue ( n =8). Data was shown as mean±standard error of the mean. Ctrl, control; DM, diabetes mellitus; IgG, immunoglobulin G; PBS, phosphate buffered saline; rIL33, recombinant IL33; NS, no significant. a P <0.05, b P <0.01.

Journal: Diabetes & Metabolism Journal

Article Title: Interleukin 33 Promotes Liver Sinusoidal Endothelial Cell Dysfunction and Hepatic Fibrosis in Diabetic Mice

doi: 10.4093/dmj.2024.0532

Figure Lengend Snippet: Interleukin 33 (IL33) promotes liver sinusoidal endothelial cell (LSEC) dysfunction in diabetic mice. (A) Representative scanning electron micrographs of livers of mice. Scale bar, 5 μm. (B) Hepatic nitric oxide (NO) levels ( n =8). (C, D) Representative immunofluorescent images and analysis of lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) and CD31 of liver sections ( n =4) (scale bar, 100 μm). (E) Relative mRNA abundance of vascular cell adhesion molecule 1 (Vcam1), intercellular adhesion molecule 1 (Icam1), nitric oxide synthase 2 (Nos2), endothelin 1 (Edn-1), nitric oxide synthase 3 (Nos3), and KLF transcription factor 2 (Klf2) of liver tissue ( n =8). Data was shown as mean±standard error of the mean. Ctrl, control; DM, diabetes mellitus; IgG, immunoglobulin G; PBS, phosphate buffered saline; rIL33, recombinant IL33; NS, no significant. a P <0.05, b P <0.01.

Techniques: Control, Saline, Recombinant

Interleukin 33 (IL33) amplifies palmitic acid and high glucose-induced liver sinusoidal endothelial cell (LSEC) dysfunction in vitro. (A) Representative fluorescent images of adhered monocytes (upper), and microscopy images of reactive oxygen species (ROS) in LSEC (lower). (B) Analysis of adherent monocytes (upper) and average fluorescent intensity of ROS (lower) ( n =4) (scale bar, 25 μm). (C) Nitric oxide (NO) levels of culture medium from LSEC administrated with recombinant IL33 in vitro (0, 1, 5, 10, 50, or 100 ng/mL) for 24 and 48 hours in the presence of palmitic acid (PA) plus high glucose (PAHG; 0.2 mM PA and 30 mM glucose) ( n =4). (D) Schematic representation of Transwell coculture system. (E, F) Representative fluorescent images and analysis of alpha smooth muscle actin (αSMA) in LX-2 co-cultured with LSEC ( n =4) (scale bar, 25 μm). (G) Western blot images and quantification of suppression of tumorigenicity 2 (ST2) from L02 cells transfected with small interfering RNA targeting ST2 (siST2) or scramble RNA. (H) Representative fluorescent images of adhered monocytes (upper), and microscopy images of ROS in LSEC (lower) after transfected with siST2 or scramble RNA ( n =3) (scale bar, 25 μm). (I) Representative fluorescent images and analysis of αSMA in LSEC transfected with siST2 or scramble RNA ( n =3) (scale bar, 25 μm). (J) Representative fluorescent images and analysis of αSMA in LX-2 cultured with culture medium collected from LSECs transfected with siST2 or scramble RNA ( n =3) (scale bar, 25 μm). Data was shown as mean±standard error of the mean. NG, normal glucose; NS, no significant; HSC, hepatic stellate cell; DAPI, 4’,6-diamidino-2-phenylindole; wt, wild-type; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. a P <0.05, b P <0.01.

Journal: Diabetes & Metabolism Journal

Article Title: Interleukin 33 Promotes Liver Sinusoidal Endothelial Cell Dysfunction and Hepatic Fibrosis in Diabetic Mice

doi: 10.4093/dmj.2024.0532

Figure Lengend Snippet: Interleukin 33 (IL33) amplifies palmitic acid and high glucose-induced liver sinusoidal endothelial cell (LSEC) dysfunction in vitro. (A) Representative fluorescent images of adhered monocytes (upper), and microscopy images of reactive oxygen species (ROS) in LSEC (lower). (B) Analysis of adherent monocytes (upper) and average fluorescent intensity of ROS (lower) ( n =4) (scale bar, 25 μm). (C) Nitric oxide (NO) levels of culture medium from LSEC administrated with recombinant IL33 in vitro (0, 1, 5, 10, 50, or 100 ng/mL) for 24 and 48 hours in the presence of palmitic acid (PA) plus high glucose (PAHG; 0.2 mM PA and 30 mM glucose) ( n =4). (D) Schematic representation of Transwell coculture system. (E, F) Representative fluorescent images and analysis of alpha smooth muscle actin (αSMA) in LX-2 co-cultured with LSEC ( n =4) (scale bar, 25 μm). (G) Western blot images and quantification of suppression of tumorigenicity 2 (ST2) from L02 cells transfected with small interfering RNA targeting ST2 (siST2) or scramble RNA. (H) Representative fluorescent images of adhered monocytes (upper), and microscopy images of ROS in LSEC (lower) after transfected with siST2 or scramble RNA ( n =3) (scale bar, 25 μm). (I) Representative fluorescent images and analysis of αSMA in LSEC transfected with siST2 or scramble RNA ( n =3) (scale bar, 25 μm). (J) Representative fluorescent images and analysis of αSMA in LX-2 cultured with culture medium collected from LSECs transfected with siST2 or scramble RNA ( n =3) (scale bar, 25 μm). Data was shown as mean±standard error of the mean. NG, normal glucose; NS, no significant; HSC, hepatic stellate cell; DAPI, 4’,6-diamidino-2-phenylindole; wt, wild-type; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. a P <0.05, b P <0.01.

Techniques: In Vitro, Microscopy, Recombinant, Cell Culture, Western Blot, Transfection, Small Interfering RNA

Interleukin 33 (IL33) drives liver sinusoidal endothelial cell (LSEC) dysfunction by blocking autophagy in vitro. (A) Western blot and measurement for light chain 3 (LC3) and p62 of LSEC ( n =4). (B) Nitric oxide (NO) levels of culture medium from LSEC cultured with different administration ( n =4). (C) Representative fluorescent images of adhered monocytes (upper), and microscopy images of reactive oxygen species (ROS) in LSEC (lower) (scale bar, 25 μm). (D) Analysis of adherent monocytes number (left) ( n =4) and average fluorescent intensity of ROS in LSEC (right) ( n =4). (E, F) Representative fluorescent images and analysis of alpha smooth muscle actin (αSMA) in LX-2 (scale bar, 25 μm). Data was shown as mean±standard error of the mean. PAHG, palmitic acid plus high glucose; Rapa, rapamycin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NG, normal glucose; NS, no significant; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01.

Journal: Diabetes & Metabolism Journal

Article Title: Interleukin 33 Promotes Liver Sinusoidal Endothelial Cell Dysfunction and Hepatic Fibrosis in Diabetic Mice

doi: 10.4093/dmj.2024.0532

Figure Lengend Snippet: Interleukin 33 (IL33) drives liver sinusoidal endothelial cell (LSEC) dysfunction by blocking autophagy in vitro. (A) Western blot and measurement for light chain 3 (LC3) and p62 of LSEC ( n =4). (B) Nitric oxide (NO) levels of culture medium from LSEC cultured with different administration ( n =4). (C) Representative fluorescent images of adhered monocytes (upper), and microscopy images of reactive oxygen species (ROS) in LSEC (lower) (scale bar, 25 μm). (D) Analysis of adherent monocytes number (left) ( n =4) and average fluorescent intensity of ROS in LSEC (right) ( n =4). (E, F) Representative fluorescent images and analysis of alpha smooth muscle actin (αSMA) in LX-2 (scale bar, 25 μm). Data was shown as mean±standard error of the mean. PAHG, palmitic acid plus high glucose; Rapa, rapamycin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NG, normal glucose; NS, no significant; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01.

Techniques: Blocking Assay, In Vitro, Western Blot, Cell Culture, Microscopy

Interleukin 33 (IL33) elicits liver sinusoidal endothelial cell (LSEC) dysfunction by activating the extracellular signal-regulated kinase 1 (ERK1)/mitogen-activated protein kinase (MAPK) pathway. (A, B) Western blot and measurement for p-ERK, ERK, light chain 3 (LC3), and p62 of LSEC ( n =3). (C) Nitric oxide (NO) levels of culture medium from LSEC cultured with different administration ( n =4). (D) Representative fluorescent images and analysis of adhered monocytes (upper), and microscopy images of reactive oxygen species (ROS) in LSEC (lower) (scale bar, 25 μm). (E) Representative fluorescent images and analysis of alpha smooth muscle actin (αSMA) in LX-2 cultured with culture medium collected from different groups of LSECs ( n =4) (scale bar, 25 μm). (F) Representative fluorescent images and analysis of αSMA in LSEC cultured with different administration ( n =4) (scale bar, 25 μm). Data was shown as mean±standard error of the mean. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NG, normal glucose; NS, no significant; PAHG, palmitic acid plus high glucose; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01.

Journal: Diabetes & Metabolism Journal

Article Title: Interleukin 33 Promotes Liver Sinusoidal Endothelial Cell Dysfunction and Hepatic Fibrosis in Diabetic Mice

doi: 10.4093/dmj.2024.0532

Figure Lengend Snippet: Interleukin 33 (IL33) elicits liver sinusoidal endothelial cell (LSEC) dysfunction by activating the extracellular signal-regulated kinase 1 (ERK1)/mitogen-activated protein kinase (MAPK) pathway. (A, B) Western blot and measurement for p-ERK, ERK, light chain 3 (LC3), and p62 of LSEC ( n =3). (C) Nitric oxide (NO) levels of culture medium from LSEC cultured with different administration ( n =4). (D) Representative fluorescent images and analysis of adhered monocytes (upper), and microscopy images of reactive oxygen species (ROS) in LSEC (lower) (scale bar, 25 μm). (E) Representative fluorescent images and analysis of alpha smooth muscle actin (αSMA) in LX-2 cultured with culture medium collected from different groups of LSECs ( n =4) (scale bar, 25 μm). (F) Representative fluorescent images and analysis of αSMA in LSEC cultured with different administration ( n =4) (scale bar, 25 μm). Data was shown as mean±standard error of the mean. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; NG, normal glucose; NS, no significant; PAHG, palmitic acid plus high glucose; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01.

Techniques: Western Blot, Cell Culture, Microscopy

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human il33 (R&D Systems)

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Enzyme-linked Immunosorbent Assay:

Incubation:

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Co-Culture Assay:

Immunohistochemistry:

Western Blot:

Immunofluorescence:

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